An improved method for partial restriction digestion of ultraviolet irradiated DNA
نویسندگان
چکیده
منابع مشابه
Block of cleavage of ultraviolet-irradiated DNA with restriction endonucleases.
pBR322/Ultraviolet irradiation/Restriction endonuclease/Pyrimidine dimer Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restric tion endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), a...
متن کاملA novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP.
Partial digestion of DNA fragments is a standard procedure for subcloning analysis and for generating restriction maps. We have developed a novel method to generate a partial digestion for any DNA fragment that can be amplified by PCR. The method involves the incorporation of 5-methyl-dCTP into the PCR product to protect most of the restriction sites. As a result, complete digestion of the modi...
متن کاملDigestion of DNA with restriction endonucleases.
Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. The first method presented in this unit is the cleavage of a single DNA sample with a single restriction endonuclease. A...
متن کاملAn improved HPLC method for determination of colocynthin in colocynth
Background and objectives: Colocynthin is the major active secondary metabolite of colocynth, Citrullus colocynthis (L.) Schrad, which has been used in traditional and ethno medicine of many countries. It could be considered as an active marker for quality control of colocynth and its herbal products. Analysis and standardization of colocynth and its herbal preparations are a critical...
متن کاملMammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
Mitochondrial forms of uracil DNA glycosylase and UV endonuclease have been purified and characterized from the mouse plasmacytoma cell line, MPC-11. As in other cell types, the mitochondrial uracil DNA glycosylase has properties very similar to those of the nuclear enzyme, although in this case the mitochondrial activity was also distinguishable by extreme sensitivity to dilution. Three mitoch...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.14.4288